A generic plant RNA isolation method suitable for RNA-Seq and suppression subtractive hybridization

作者：Zhu, Y Q; Wu, W J; Xiao, H W; Chen, H B; Zheng, Y; Zhang, Y J; Wang, H X; Huang, L Q^*

来源：Genetics and Molecular Research, 2013, 12(4): 5537-5546.

DOI：10.4238/2013.November.18.4

• Plant RNA
• Isolation
• Polyphenols
• Polysaccharides
• RNA-Seq

A recently developed revolutionary approach to transcriptomics, RNA-Seq, and suppression subtractive hybridization are powerful tools for gene expression research. However, currently, the difficulty of isolating high-quality RNAs from plant tissues bearing abundant complex polysaccharides, polyphenolics, and secondary metabolites is a serious problem that not only limits the application of these technologies but also hinders studies dealing with RNA in general. We have developed a consistent protocol to prepare highly intact and pure RNAs from tissues of a variety of field-grown plant species, with high yields, in 2 to 3 h. Additionally, this method can be readily applied to mammalian, yeast, and bacterial cells.

• 出版日期2013
• 单位清华大学