A new "off-on" fluorescent probe for Pd2+ in aqueous solution and live-cell based on spirolactam ring-opening reaction

作者：Huang Qi; Zhou Yanmei^*; Zhang Qingyou; Wang Enze; Min Yinghao; Qiao Han; Zhang Junli; Ma Tongsen

来源：Sensors and Actuators B: Chemical , 2015, 208: 22-29.

DOI：10.1016/j.snb.2014.11.012

• Rhodamine B
• Fluorescent sensor
• Pd2+
• Aqueous solution
• Live-cell imaging

Based on spirolactam ring-opening reaction, a chromogenic and "off-on" fluorescent sensor for determination of palladium (II) was developed in this paper. The novel sensor was easily synthesized from rhodamine B hydrazide and diacetyl monoxime named RBM. The structure of the sensor has been characterized by ESI-MS and NMR. EDTA-adding experiment indicated that the binding of chemosensor RBM and Pd2+ was chemically reversible. The fluorescence intensity and absorbance of the sensor showed a good linearity with the concentration of Pd2+ in the range of 0-15 mu M and 0-17 mu M, respectively, with detection limits of 0.015 mu M and 0.200 mu M. Moreover, it has been successfully applied to detect Pd2+ in palladium-containing catalyst, natural water samples and imaging of Pd2+ in living cells with satisfying results. The study indicated that this novel sensor could be an attractive approach to detect Pd2+ in environmental systems and biological systems.

• 出版日期2015-3-1
• 单位河南大学