Decellularized, xenogeneic small-diameter arteries: Transition from a muscular to an elastic phenotype in vivo

作者：Bergmeister Helga; Plasenzotti Roberto; Walter Ingrid; Plass Christian; Bastian Fabienne; Rieder Erwin; Sipos Wolfgang; Kaider Alexandra; Losert Udo; Weigel Guenter^*

来源：Journal of Biomedical Materials Research Part B: Applied Biomaterials , 2008, 87B(1): 95-104.

DOI：10.1002/jbm.b.31074

• animal model
• tissue engineering
• vascular graft
• histology

Reports regarding the biocompatibility of xenogeneic, decellularized bioprosthetic implants differ between bioinertness and complete graft degradation. We investigated heparin-crosslinked and nonheparinized, xenogeneic vascular substitutes in a rat model. Porcine arteries (15 X 1.5 mm) were decellularized by multistep detergent and enzymatic techniques, which were followed by heparin-crosslinking in 50% of the implants. Prostheses were implanted into the abdominal aorta of 76 rats for I day and up to 6 months. Retrieved specimens were evaluated by histology, immunohistochemistry, laser scanning, and scanning electron microscopy. Graft patency did not differ between groups (97.3%). Heparinized grafts showed a statistically significant lower rate of aneurysm formation (P = 0.04 %). Implants revealed infiltration with granulocytes and macrophages up to 3 months. Recellularization with endothelial cells and myoribroblasts was detectable within I month. After 6 months elastin biosynthesis and complete graft remodeling toward an elastic vessel was evident. These results indicate that temporary inflammation does not interfere with long-term vascular remodeling.

• 出版日期2008-10