A correlation between polyomavirus JC virus quantification and genotypes in renal transplantation

作者：Yin Wen Yao; Lu Ming Chi; Lee Ming Che; Liu Su Chin; Lin Teng Yi; Lai Ning Sheng^*

来源：American Journal of Surgery, 2010, 200(1): 53-58.

DOI：10.1016/j.amjsurg.2009.03.017

• Capillary electrophoresis
• JC virus
• Polyomavirus
• Real-time polymerase chain reaction
• Transplant patients

OBJECTIVE: To determine whether the John Cunningham virus (JCV) viral load and the multigenotypes in viruria are correlated with transplant patients.
METHODS: The urine of 60 renal transplant patients and 60 healthy controls were screened. We used quantitative real-time polymerase chain reaction and capillary electrophoresis to assess viral load and genotype respectively.
RESULTS: The incidence of viruria and viral load were higher in transplant patients with P = .0092 and P = .0094, respectively. The incidence of different genotype in transplant patients versus controls was 8.3% versus 13.3% for single genotype, 26.7% versus 5% for 2 genotypes, and 5% versus 0% for multigenotypes (P = .0004). The incidence of more than 2 genotypes was high in people with a high viral load and closely related with the transplant patients (P = .007).
CONCLUSIONS: Not only viral load but also genotypes are important as a screening parameter to understand the immune milieu of the patients to prevent subsequent complications like polyomavirus nephropathy, infection, and malignancy.

• 出版日期2010-7