Fast Production of Diacylglycerol in a Solvent Free System via Lipase Catalyzed Esterification Using a Bubble Column Reactor

作者：Liu, Manman; Fu, Junning; Teng, Yinglai; Zhang, Zhen; Zhang, Ning^*; Wang, Yong^*

来源：Journal of the American Oil Chemists' Society, 2016, 93(5): 637-648.

DOI：10.1007/s11746-016-2804-y

• Bubble column reactor
• Diacylglycerols
• Lipase
• Esterification
• Crystal polymorphism

In this study, diacylglycerols (DAG) were synthesized rapidly (similar to 30 min) in a solvent-free system via esterification of glycerol with fatty acids (FA, the mixture of 60 wt% palm oil deodorizer distillate and 40 wt% oleic acid) catalyzed by Lipozyme 435 (Novozymes A/S, Copenhagen, Denmark) using a bubble column reactor. The content of DAG, monoacylglycerols (MAG), triacylglycerols (TAG) and free fatty acids (FFA) in the crude product were 57.94 +/- 1.60 wt%, 24.68 +/- 2.08 wt%, 2.67 +/- 1.72 wt% and 14.69 +/- 1.22 wt%, respectively under the selected conditions, which were enzyme load of 5.0 wt%, glycerol/FA mole ratio of 7.5, initial water content of 2.5 wt%, reaction temperature of 60 degrees C, reaction time of 30 min and N-2 gas flow of 10.6 cm min(-1). The final product containing 91.30 +/- A 1.10 wt% of DAG was obtained by one-step molecular distillation at 200 degrees C. The reusability of Lipozyme 435 was investigated by evaluating the esterification degree (ED) and the DAG content in the crude products in 30 successive runs. The enzyme retained 95.10 % of its original activity during 30 successive runs according to comparison of the ED. The new process showed a very high efficiency in production of DAG with a high purity. The ratio of positional isomers 1,3-DAG to 1,2 -DAG was 2:1 in the final product. The certain plasticity (melting point of 44 degrees C) and content of unsaturated fatty acids made the product a valuable food ingredient.

• 出版日期2016-5
• 单位暨南大学