Optimization of multi-stage countercurrent extraction of antioxidants from Ginkgo biloba L. leaves

作者：Yu, Chen-huan^*; Chen, Jing; Xiong, Yao-kang; Li, Xiao-xian; Dai, Xiao-yan; Shi, Chang-cheng

来源：Food and Bioproducts Processing, 2012, 90(C2): 95-101.

DOI：10.1016/j.fbp.2011.05.003

• Orthogonal array design
• Antioxidant
• Flavonoids
• Quercetin
• Total phenolics

Multi-stage countercurrent extraction (MCE) as a novel extraction technique was used to extract antioxidants from Ginkgo biloba leaves. Orthogonal array design (OAD) was employed to optimize the ratio of 60% ethanol to raw material (8-16 mL/g), extraction time (30-60 min) and extraction temperature (60-80 degrees C) to obtain a high yield of antioxidants from G. biloba leaves by MCE. The optimum conditions were a ratio of 60% ethanol to raw material of 16 mL/g and extraction time of 30 min at 80 degrees C. Under these conditions, the yields of flavonoids and total phenolics were 1.74% and 2.42%, respectively, and DPPH radicals scavenging activity of the extract was 89.97%. Compared with heat-reflux extraction, MCE had obvious advantages of less extraction time and lower solvent and energy consumption. It may be used as a promising technique for the extraction of bioactive compounds from plant materials.

• 出版日期2012-4
• 单位浙江省医学科学院; 浙江中医药大学; 南京中医药大学