Adeno-Associated Virus Harboring Fusion Gene NT4-Ant-Shepherdin Induce Cell Death in Human Lung Cancer Cells

作者：Tang Xiaojiang; Zhou Jinsong; Wang Jiansheng; Pan Chengen^*; Yang Guangxiao; Wang Quanying

来源：Cancer Investigation, 2010, 28(5): 465-471.

DOI：10.3109/07357900903095706

• Lung Cancer
• Oncogenes
• Therapy

To further enhance anticancer effect of Shepherdin and overcome limitation of peptide therapy, recombinant adeno-associated virus (rAAV) was constructed with following strategies: therapeutic peptide secretory expression and adeno-associated virus gene transfer system. MTT assay and flow cytometric analysis revealed that rAAV harboring fusion gene NT4-Ant-Shepherdin significantly suppressed A549 cell growth in a time-dependent manner and induced apoptosis. In the infected A549 cells, survivin expression level decreased strongly while Caspase-3/7 activities increased significantly. These results indicated that rAAV harboring fusion gene NT4-Ant-Shepherdin may be a novel strategy in lung cancer peptide therapy.

• 出版日期2010-6
• 单位西安交通大学