Appropriate expression of imprinted genes on mouse chromosome 12 extends development of bi-maternal embryos to term

作者：Kawahara Manabu; Wu Qiong; Ferguson Smith Anne C; Kono Tomohiro^*

来源：FEBS LETTERS, 2007, 581(27): 5178-5184.

DOI：10.1016/j.febslet.2007.10.004

• H19
• Igf2
• genomic imprinting
• development mouse

Recently, we reported that the restored regulation of imprinted gene expression from two regions - H19 differentially methylated region (H19-DMR) and intergenic germline-derived DMR (IG-DMR) - is sufficient for accomplishing full-term development in mice. In the present study, we determined the developmental ability of the bi-maternal embryos (BMEs) containing the non-growing oocyte genome with the IG-DMR deletion (ng(Delta ch12)) and fully-grown (fg) oocyte genome. Foetuses derived from ng(Delta ch12)/fg BMEs were alive at E19.5 but could not survive further. Comparison with BMEs derived from Igf2+/- ng/fg genomes suggests that bi-allelic H19 expression might be involved in foetal development.

• 出版日期2007-11-13