Airway Contractility in the Precision-Cut Lung Slice after Cryopreservation

作者：Rosner Sonia R; Ram Mohan Sumati; Paez Cortez Jesus R; Lavoie Tera L; Dowell Maria L; Yuan Lei; Ai Xingbin; Fine Alan; Aird William C; Solway Julian; Fredberg Jeffrey J; Krishnan Ramaswamy^*

来源：American Journal of Respiratory Cell and Molecular Biology, 2014, 50(5): 876-881.

DOI：10.1165/rcmb.2013-0166MA

• precision-cut lung slice
• cryopreservation
• airway contractility
• airway smooth muscle

An emerging tool in airway biology is the precision-cut lung slice (PCLS). Adoption of the PCLS as a model for assessing airway reactivity has been hampered by the limited time window within which tissues remain viable. Here we demonstrate that the PCLS can be frozen, stored long-term, and then thawed for later experimental use. Compared with the never-frozen murine PCLS, the frozen-thawed PCLS shows metabolic activity that is decreased to an extent comparable to that observed in other cryopreserved tissues but shows no differences in cell viability or in airway caliber responses to the contractile agonist methacholine or the relaxing agonist chloroquine. These results indicate that freezing and long-term storage is a feasible solution to the problem of limited viability of the PCLS in culture.

• 出版日期2014-5