科研之友
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摘要
An improved method was developed for the determination of the gas-phase free radicals in mainstream cigarette smoke. A trap device for the gas-phase free radicals was developed, which could be easily attached to and removed from a commercial linear smoking machine. N-t-Butyl-ci-phenylnitrone ( PBN) was used to trap the gas-phase free radicals and the formed adduct was detected with EPR technique. The adduct was immediately frozen in liquid nitrogen for degassing and stabilization of the adduct. 2,2,6,6-Tetramethyl-piperi -l-oxy(TEMPO) and strong pitch was used as calibration samples to quantitatively analyze the gas-phase I free radicals. The sampling, conditioning and selection of cigarettes in the test were accordant with ISO 4387. It was proved that the established method was highly sensitive, reproducible, practicable and accurate for the detection of the gas-phase free radicals in routine tests.
