Antibacterial and anti-biofilm effects of cathelicidin peptides against pathogens isolated from cystic fibrosis patients

作者：Pompilio A; Scocchi M; Pomponio S; Guida F; Di Primio A; Fiscarelli E; Gennaro R; Di Bonaventura G^*

来源：Peptides, 2011, 32(9): 1807-1814.

DOI：10.1016/j.peptides.2011.08.002

• Antimicrobial peptide
• Cathelicidin
• Cystic fibrosis
• Antibiotic
• Multidrug-resistance

Six different cathelicidin-derived peptides were compared to tobramycin for antibacterial and anti-biofilm effects against S. aureus, P. aeruginosa, and S. maltophilia strains isolated from cystic fibrosis patients. Overall, SMAP-29, BMAP-28, and BMAP-27 showed relevant antibacterial activity (MIC(50) 4-8 mu g/ml), and in some cases higher than tobramycin. In contrast, indolicidin, LL-37. and Bac7(1-35) showed no significant antimicrobial activity (MIC(50) > 32 mu g/ml). Killing kinetics experiments showed that in contrast to tobramycin the active cathelicidin peptides exert a rapid bactericidal activity regardless of the species tested. All three peptides significantly reduced biofilm formation by S. maltophilia and P. aeruginosa strains at 1/2 x MIC, although at a lower extent than tobramycin. In addition, BMAP-28, as well as tobramycin, was also active against S. aureus biofilm formation. Preformed biofilms were significantly affected by bactericidal SMAP-29, BMAP-27 and BMAP-28 concentrations, although at a lesser extent than tobramycin. Overall, our results indicate the potential of some cathelicidin-derived peptides for the development of novel therapeutic agents for cystic fibrosis lung disease.

• 出版日期2011-9