Determination of absorption coefficients for chlorophyll a, phycocyanin, mineral matter and CDOM from three central Indiana reservoirs

作者：Nguy Robertson Anthony^*; Li Lin; Tedesco Lenore P; Wilson Jeffrey S; Soyeux Emmanuel

来源：Journal of Great Lakes Research, 2013, 39: 151-160.

DOI：10.1016/j.jglr.2013.04.004

• Remote sensing
• Bio-optical modeling
• Cyanobacteria
• Suspended sediments

Absorption coefficients are used in semi-empirical and bio-optical models to estimate various optically active constituents in water. This study determines the mass-specific absorption coefficients of chlorophyll a (a*(chl)), phycocyanin (a*(pc)), minerals (a*(m)), and chromophoric dissolved organic matter (CDOM; a*(CDOM)) and the absorption coefficient for CDOM (a(CDOM)) for three central Indiana reservoirs. Absorption spectra were collected using a Shimadzu spectrophotometer equipped with a Spectralon coated integrating sphere. Quantitative measurements from either fluorometric (chlorophyll a, phycocyanin, CDOM) or weight-based (mineral) measurements were used to determine the mass-specific absorption coefficients tested in this study and an exponential function was used to estimate aCDOM. While a*chl showed minimum variability between reservoirs, a*(pc), a*(m), a*(CDOM) and a(CDOM) showed statistically significant differences between reservoirs; however, for a*(m), there was greater variation within each reservoir. Variability in a*(CDOM) and a(CDOM) between reservoirs was likely due to different inflows. All absorption coefficients fell within previously published ranges for each constituent examined, with the exception of a*(pc). Differences in a*(pc) were likely due to different techniques used in extracting and estimating phycocyanin.

• 出版日期2013