摘要

Background/Aims: Dendritic cells (DCs) are professional antigen-presenting cells responsible for initiating of immune response. However, because of immune tolerance, it is difficult to induce long-term tumor-specific immune response in humans. This is probably because DCs, which combine with Th2 in the Tim-1/Tim-4 pathway, will induce Th2 to proliferation. Methodology: We have transfected siRNA of FG-CC' gene into DCs stimulated by gastric cancer lysate (lysate-FG-CC-siRNA group), FG-CC-siRNA will block FG-CC' loop, which plays an important role in interaction between Tim-1 and Tim-4. Their potential effect on gastric cancer immunotherapy is assessed by an experimental model. Results: It was observed that lysate-FG-CC-siRNA had the strongest ability of adjusting balance on the Th1/Th2, as a result, these DCs can inhibit gastric cancer growth. In order to test the ability of FG-CC-siRNA DCs to inhibit tumor growth, we immunized mice subcutaneously with DCs transfected with FG-CC-siRNA plus tumor antigen. Compared with the control group, a significant inhibition of tumor growth was obvious for the group of lysate-FG-CC-siRNA DC. Conclusions: We have shown that FG-CC-siRNA blocks FG-CC' loop and significantly enhances the anti-tumor immunity in vitro and in vivo.

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