Inward rectifier K+ channel and T-type Ca2+ channel contribute to enhancement of GABAergic transmission induced by beta(1)-adrenoceptor in the prefrontal cortex

作者：Luo, Fei^*; Zheng, Jian; Sun, Xuan; Tang, Hua

来源：Experimental Neurology, 2017, 288: 51-61.

DOI：10.1016/j.expneurol.2016.11.007

• beta(1)-Adrenoceptor
• GABAergic transmission
• T-type calcium channel
• Inward rectifier K+ channel
• Prefrontal cortex

The functions of prefrontal cortex (PFC) are sensitive to norepinephrine (NE). Endogenously released NE influences synaptic transmission through activation of different subtypes of adrenergic receptors in PFC including alpha(1), alpha(2), beta(1) or beta(2)-adrenoceptor. Our recent study has revealed that beta(1)-adrenoceptor (beta(1)-AR) activation modulates glutamatergic transmission in the PFC, whereas the roles of beta(1)-AR in GABAergic transmission are elusive. In the current study, we probed the effects of the beta(1)-AR agonist dobutamine (Dobu) on GABAergic transmission onto pyramidal neurons in the PFC of juvenile rats. Dobu increased both the frequency and amplitude of miniature IPSC5 (mIPSCs). Ca2+ influx through T-type voltage-gated Ca2+ channel was required for Dobu-enhanced mIPSC frequency. We also found that Dobu facilitated GABA release probability and the number of releasable vesicles through regulating T-type Ca2+ channel. Dobu depolarized GABAergic fast-spiking (FS) interneurons with no effects on the firing rate of action potentials (APs) of interneurons. Dobu-induced depolarization of FS inter neurons required inward rectifier K+ channel (Kir). Our results suggest that Dobu increase GABA release via inhibition of Kir, which further depolarizes FS interneurons resulting in Ca2+ influx via T-type Ca2+ channel.

• 出版日期2017-2
• 单位南昌大学