Multifunctional mesoporous silica nanoparticles as labels for the preparation of ultrasensitive electrochemical immunosensors

作者：Yang Minghui; Li He; Javadi Alireza; Gong Shaoqin^*

来源：Biomaterials, 2010, 31(12): 3281-3286.

DOI：10.1016/j.biomaterials.2010.01.033

• Enzyme
• Electrochemistry
• Mesoporous silica nanoparticle
• Immunosensor

Labels based on mesoporous silica nanoparticles (MSN) loaded with mediator thionine (TH), enzyme horseradish peroxidase (HRP) and secondary anti-human IgG antibody (Ab(2)) were developed in order to improve the sensitivity and detection limit of an amperometric immunosensor. The sensitivity of the sandwich-type immunosensor using MSN-TH-HRP-Ab(2) as labels for human IgG detection was about 100 times higher than that using either MSN-TH-Ab(2) or MSN-HRP-Ab(2) as labels, indicating the high catalytic efficiency of HRP in the presence of mediator TH toward H(2)O(2). The immunosensor using MSN-TH-Ab(2) as labels exhibited a high sensitivity and showed a linear response within the range of 0.01-10 ng/mL human IgG. It also showed good reproducibility and selectivity and acceptable stability. These labels for immunosensors may provide many potential applications for the detection of different biomolecules.

• 出版日期2010-4