A novel protein extraction method for identification of mycobacteria using MALDI-ToF MS

作者：Adams La'Tonzia L^*; Salee Parichat; Dionne Kim; Carroll Karen; Parrish Nicole

来源：Journal of Microbiological Methods, 2015, 119: 1-3.

DOI：10.1016/j.mimet.2015.09.010

• Mycobacterium
• Protein extraction
• MALDI-ToF MS

Commercial extraction methods for identification of mycobacteria using MALDI-ToF MS are laborious and time consuming. We have developed a novel extraction method which utilizes a bead beater and zirconia/silica beads to significantly shorten the existing protocol. This novel method provides a more rapid extraction of mycobacteria versus the commercial standard.

• 出版日期2015-12