Determination of Optical Coefficients and Fractal Dimensional Parameters of Cancerous and Normal Prostate Tissues

作者：Pu Yang; Wang Wubao; AL Rubaiee Mohammad; Gayen Swapan Kumar; Xu Min^*

来源：Applied Spectroscopy, 2012, 66(7): 828-834.

DOI：10.1366/11-06471

• Prostate tissue
• Prostate cancer detection
• Biomedical imaging
• Absorption coefficient
• Scattering coefficient
• Reduced scattering coefficient
• Anisotropy factor
• Near-infrared imaging
• NIR spectroscopy
• Diffusion
• Mie theory
• Fractal tissue model
• Transmission imaging

Optical extinction and diffuse reflection spectra of cancerous and normal prostate tissues in the 750 to 860 nm spectral range were measured. Optical extinction measurements using thin ex vivo prostate tissue samples were used to determine the scattering coefficient (mu(s)), while diffuse reflection measurements using thick prostate tissue samples were used to extract the absorption coefficient (mu(a)) and the reduced scattering coefficient (mu(s)'). The anisotropy factor (g) was obtained using the extracted values of mu(s) and mu(s)'. The values of fractal dimension (D-f) of cancerous and normal prostate tissues were obtained by fitting to the wavelength dependence of mu(s)'. The number of scattering particles contributing to mu(s) as a function of particle size and the cutoff diameter d(max) as a function of g were investigated using the fractal soft tissue model and Mie theory. Results show that d(max) of the normal tissue is larger than that of the cancerous tissue. The cutoff diameter d(max) is observed to agree with the nuclear size for the normal tissues and the nucleolar size for the cancerous tissues. Transmission spectral polarization imaging measurements were performed that could distinguish the cancerous prostate tissue samples from the normal tissue samples based on the differences between their absorption and scattering parameters.

• 出版日期2012-7