Dynamic molecular monitoring of retina inflammation by in vivo Raman spectroscopy coupled with multivariate analysis

作者：Marro Monica; Taubes Alice; Abernathy Alice; Balint Stephan; Moreno Beatriz; Sanchez Dalmau Bernardo; Martinez Lapiscina Elena H; Amat Roldan Ivan; Petrov Dmitri; Villoslada Pablo^*

来源：Journal of Biophotonics, 2014, 7(9): 724-734.

DOI：10.1002/jbio.201300101

• Raman spectroscopy
• multivariate curve resolution
• molecular imaging
• neuroinflammation
• retina ganglion cells
• Multiple Sclerosis

Retinal tissue is damaged during inflammation in Multiple Sclerosis. We assessed molecular changes in inflamed murine retinal cultures by Raman spectroscopy. Partial Least Squares-Discriminant analysis (PLS-DA) was able to classify retina cultures as inflamed with high accuracy. Using Multivariate Curve Resolution (MCR) analysis, we deconvolved 6 molecular components suffering dynamic changes along inflammatory process. Those include the increase of immune mediators (Lipoxygenase, iNOS and TNF alpha), changes in molecules involved in energy production (Cytochrome C, phenylalanine and NADH/NAD+) and decrease of Phosphatidylcholine. Raman spectroscopy combined with multivariate analysis allows monitoring the evolution of retina inflammation.

• 出版日期2014-9