Novel, closely related, white spot syndrome virus (WSSV) genotypes from Madagascar, Mozambique and the Kingdom of Saudi Arabia

作者：Tang Kathy F J^*; Le Groumellec Marc; Lightner Donald V

来源：Diseases of Aquatic Organisms, 2013, 106(1): 1-6.

DOI：10.3354/dao02645

• WSSV genotyping
• Variable number of tandem repeats
• VNTR analysis
• Variable-length deletion
• Africa
• Saudi Arabia

White spot syndrome virus (WSSV) is highly pathogenic to penaeid shrimp and has caused significant economic losses in the aquaculture industry around the world. During 2010 to 2012, WSSV caused severe mortalities in cultured penaeid shrimp in Saudi Arabia, Mozambique and Madagascar. To investigate the origins of these WSSV, we performed genotyping analyses at 5 loci: the 3 open reading frames (ORFs) 125, 94 and 75, each containing a variable number of tandem repeats (VNTR), and deletions in the 2 variable regions, VR14/15 and VR23/24. We categorized the WSSV genotype as {N-125, N-94, N-75, Delta X-14/15, Delta X-23/24} where N is the number of repeat units in a specific ORF and Delta X is the length (base pair) of deletion within the variable region. We detected 4 WSSV genotypes, which were characterized by a full-length deletion in ORF94/95, a relatively small ORF75 and one specific deletion length in each variable region. There are 2 closely related genotypes in these 3 countries: {6(125), del(94), 3(75), Delta 5950(14/15), Delta 10971(23/24)} and {7(125), del(94), 3(75), Delta 5950(14/15), Delta 10971(23/24)}, where del is the full-length ORF deletion. In Saudi Arabia, 2 other related types of WSSV were also found: {6(125), 7(94), 3(75), Delta 5950(14/15), Delta 10971(23/24)} and {8(125), 13(94), 3(75), Delta 5950(14/15), Delta 10971(23/24)}. The identical patterns of 3 loci in these 4 types indicate that they have a common lineage, and this suggests that the WSSV epidemics in these 3 countries were from a common source, possibly the environment.

• 出版日期2013-9-24