Novel genes and cellular pathways related to infection with adenovirus-36 as an obesity agent in human mesenchymal stem cells

作者：Na H N; Kim H; Nam J H^*

来源：International Journal of Obesity, 2012, 36(2): 195-200.

DOI：10.1038/ijo.2011.89

• Ad-36
• human mesenchymal stem cell
• PPAR-gamma signaling
• inflammation
• gene set enrichment analysis

Objective: The objective of this study was to investigate the molecular mechanisms underlying adenovirus-36 (Ad-36)-induced obesity by the identification of novel genes and cellular pathways. %26lt;br%26gt;Design: Viral growth, intracellular lipid accumulation and gene expression profiles were determined in human mesenchymal stem cells (hMSCs) infected with Ad-36 or Ad-2. A microarray assay and gene set enrichment analysis (GSEA) were performed to assess alterations in global gene expression profiles. %26lt;br%26gt;Results: Ad-36, but not Ad-2, induced lipid accumulation and upregulated adipogenesis-related genes. There was no difference in viral growth between Ad-36 infection and Ad-2 infection in hMSCs. GSEA revealed that Ad-36 infection was more frequently associated with activation of novel pathways, including the PPAR-gamma signaling pathway, and inflammation compared with Ad-2 infection, raising the possibility that these pathways may be key regulators of Ad-36-induced adipogenesis. %26lt;br%26gt;Conclusion: This study may help foster a better understanding of the roles of several cellular factors in Ad-36-induced obesity. International Journal of Obesity (2012) 36, 195-200; doi:10.1038/ijo.2011.89; published online 3 May 2011

• 出版日期2012-2