Multilayer engineered nanoliposomes as a novel tool for oral delivery of lipopeptide-based vaccines against group A Streptococcus

作者：Marasini Nirmal; Giddam Ashwini K; Ghaffar Khairunnisa A; Batzloff Michael R; Good Michael F; Skwarczynski Mariusz^*; Toth Istvan^*

来源：Nanomedicine, 2016, 11(10): 1223-1236.

DOI：10.2217/nnm.16.36

• group A Streptococcus
• lipid-core peptide
• liposomes
• mucosal immunology
• nanoparticles
• oral delivery
• peptides
• vaccines

Aim: To develop an oral nanovaccine delivery system for lipopeptide-based vaccine candidate against group A Streptococcus. Materials & methods: Lipid-core peptide-1-loaded nanoliposomes were prepared as a template and coated with opposite-charged polyelectrolytes to produce particles with size <200 nm. Efficacy of this oral nanovaccine delivery system was evaluated in mice model. Results: Polymer-coated liposomes produced significantly higher antigen-specific mucosal IgA and systemic IgG titers in comparison to vaccine formulated with a strong mucosal adjuvant upon oral immunization in mice. Moreover, high levels of systemic antibody titers were retained even at day 185 postprimary immunization. Conclusion: Efficient oral delivery platform for lipopeptide-based vaccines has been developed.

• 出版日期2016