A potent vasoactive cytolysin isolated from Scorpaena plumieri scorpionfish venom

作者：Andrich F; Carnielli J B T; Cassoli J S; Lautner R Q; Santos R A S; Pimenta A M C; de Lima M E; Figueiredo S G^*

来源：Toxicon, 2010, 56(4): 487-496.

DOI：10.1016/j.toxicon.2010.05.003

• Scorpionfish venom
• Scorpaena plumieri
• Cytolysin
• Vasoactive protein

A new vasoactive cytolytic toxin, referred to as Sp-CTx, has been purified from the venom of the scorpionfish Scorpaena plumieri by a combination of gel filtration and anion exchange chromatographies. An estimation of Sp-CTx native molecular mass, performed by size exclusion chromatography, demonstrated that it is a 121 kDa protein Further physicochemical studies revealed its glycoproteic nature and dimeric constitution, comprising subunits of approximately 65 kDa (MALDI-TOF-MS). Such protein has proved to possess a potent hemolytic activity on washed rabbit erythrocytes (EC(50) 0.46 nM), whose effect was strongly reduced after treatment with antivenom raised against stonefish venom - Synanceja trachynis (SFAV). This cross-reactivity has been confirmed by western blotting. Like S plumieri whole venom (100 mu g/mL), Sp-CTx (1-50 nM) caused a biphasic response on phenylephrine pre-contracted rat aortic rings, characterized by an endothelium- and dose-dependent relaxation phase followed by a contractile phase. The vasorelaxant activity has been abolished by L-NAME, demonstrating the involvement of nitric oxide on the response We report here the first isolation of a cytolytic/vasoactive protein fr

• 出版日期2010-9-15