Analysis of baicalein, baicalin and wogonin in Scutellariae radix and its preparation by microemulsion electrokinetic chromatography with 1-butyl-3-methylimizolium tetrafluoborate ionic liquid as additive

作者：Zhang Huige; Tian Kan; Tang Jianghong; Qi Shengda; Chen Hongli; Chen Xingguo^*; Hu Zhide

来源：Journal of Chromatography A, 2006, 1129(2): 304-307.

DOI：10.1016/j.chroma.2006.08.016

• microemulsion electrokinetic capillary chromatography
• baicalein
• baicalin
• wogonin
• ionic liquid

Microemulsion electrokinetic chromatography (MEEKC) using 1-butyl-3-methylimidazolium tetrafluoroborate (BMIM-BF4) ionic liquid (EL) as additive was developed for the analysis of baicalin, wogonin and baicalein in Scutellariae radix and its preparation. After conducting a series of optimizations, baseline separation was obtained for the analytes within 5 min under the optimum conditions (sodium dodecyl sulfate (SDS) 0.88% (m/v) ethyl acetate 0.8% (v/v) butan-1-ol 0.2% (v/v) and the buffer composition were 25% acetonitrile (v/v), 7.5 mm BMIM-BF4 and 10mM NaH2PO4, pH 8.2, applied voltage 17.5kV and detection at 254nm), the method has been successfully applied to the determination and quantification of the analytes in the extracts of S. radix (cooked), S. radix (raw) and Qingfeiyihuowan which was the preparation including S. radix.

• 出版日期2006-10-6
• 单位兰州大学