科研之友
微信
新浪微博
Facebook
分享链接
摘要
Food safety problems such as damage to immune, nervous, and endocrine systems leading to cancer and malformations have received increasing attention. To achieve the maximum residue limits, the most discussed method of high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) is widely used-with a advantage of high precision and resolution. Prothioconazole is a broad-spectrum thiocarbamate fungicide. it can rapidly metabolize to prothioconazole-desthio in different matrixes. Rapid and effective methods for the determination of prothioconazole-desthio in five kinds of different animal food were developed. Samples were extracted with acetonittile or acetonitrile/water and determined by HPLC MS/MS. The limit of detection and limit of quantification values of prothioconazole-desthio were 0.015 and 0.05 mg/kg for porcine liver and kidney, 0.0015 and 0.005 mg/kg for pork, and 0.003 and 0.01 mg/kg for eggs, together with 0.0012 and 0.004 mg/kg for milk of the detected method, respectively. A good linear regression trend can be observed in a certain concentration range for all of the animal food. At fortified levels, recoveries were between 83.6 and 105%; with relative standard deviations of 1.5-10.3%. A sample survey of 150 samples with 30 samples for each kind, of animal food across the country was conducted and found that there was no prothioconazole-desthio detected in all samples.
- 出版日期2017-3-22
- 单位中国农业大学
