An easy-to-use tool for planning and modeling a calorimetric titration

作者：Biswas Tapan^*; Tsodikov Oleg V

来源：Analytical Biochemistry, 2010, 406(1): 91-93.

DOI：10.1016/j.ab.2010.06.050

Isothermal titration calorimetry (ITC) is widely employed to measure thermodynamic properties of binding interactions between two macromolecules or a macromolecule and a small ligand. No labeling of interacting species is required for ITC, but this advantage is offset by potentially material-consuming experimental optimization complicated by an indirect readout of an ITC titration. Here we present a simple, practical, and portable spreadsheet-based tool for planning and modeling an ITC titration experiment accompanied by basic guidelines.

• 出版日期2010-11-1