Assessing acceptor substrate promiscuity of YjiC-mediated glycosylation toward flavonoids

作者：Pandey Ramesh Prasad; Gurung Rit Bahadur; Parajuli Prakash; Koirala Niranjan; Tuoi Le Thi; Sohng Jae Kyung^*

来源：Carbohydrate Research, 2014, 393: 26-31.

DOI：10.1016/j.carres.2014.03.011

• Flavonoids
• Glycosyltransferase
• Natural product glucosides
• Non-regiospecific glycosylation

The acceptor substrate promiscuity of YjiC, a UDP-glycosyltransferase from Bacillus licheniformis, was explored with seven different classes (flavonols, flavanols, flavones, flavanones, chalcone, stilbene, and isoflavonoids) of 23 flavonoid acceptors. For most of the polyphenols used in the reactions, the enzymatic bioconversion was significantly higher with the production of multiple glucosylated derivatives. This study highlights the highly flexible non-regiospecific glycosylation ability of YjiC toward polyphenolic compounds. The catalytic potential of YjiC could be useful to generate a library of natural product glucosides.

• 出版日期2014-7-1