A Novel Approach Utilizing Biofilm Time-Kill Curves To Assess the Bactericidal Activity of Ceftaroline Combinations against Biofilm-Producing Methicillin-Resistant Staphylococcus aureus

作者：Barber Katie E; Werth Brian J; McRoberts John P; Rybak Michael J^*

来源：Antimicrobial Agents and Chemotherapy, 2014, 58(5): 2989-2992.

DOI：10.1128/AAC.02764-13

Medical device infections frequently require combination therapy. Beta-lactams combined with glycopeptides/lipopeptides are bactericidal against methicillin-resistant Staphylococcus aureus (MRSA). Novel macrowell kill-curve methods tested synergy between ceftaroline or cefazolin plus daptomycin, vancomycin, or rifampin against biofilm-producing MRSA. Ceftaroline combinations demonstrated the most pronounced bacterial reductions. Ceftaroline demonstrated greatest kill with daptomycin (4.02 +/- 0.59 log(10) CFU/cm(2)), compared to combination with vancomycin (3.36 +/- 0.35 log(10) CFU/cm(2)) or rifampin (2.68 +/- 0.61 log(10) CFU/cm(2)). These data suggest that beta-lactam combinations are useful against MRSA biofilms.

• 出版日期2014-5