Determination of N-epsilon-Carboxymethyl-lysine Content in Muscle Tissues of Turbot by Gas Chromatography-Mass Spectrometry

作者：Wang Jing; Tian Sheng-Lan; Sun Wei-Hong; Lin Hong; Li Zhen-Xing^*

来源：Chinese Journal of Analytical Chemistry, 2015, 43(8): 1187-1191.

DOI：10.1016/S1872-2040(15)60847-7

• N-epsilon-Carboxymethyl-lysine
• Fishery products
• Gas chromatography-mass spectrometry
• Derivatization

A method was developed to determine the N-epsilon-carboxymethyl-lysine content in the muscle tissues of turbot using gas chromatography-mass spectrometric method. Turbot was selected as the research object, and homogenized muscle tissue samples of turbot defatted by chloroform/acetone were pretreated followed by reduction, acid hydrolysis, purifying and derivatization (esterification and acylation). The ion of m/z 392 was selected as a quantitative ion, and the ions of m/z 392, 365, 333, and 305 were selected as qualitative ions in the selected ion monitoring mode. The range of the linear calibration curve of the N-epsilon-carboxymethyl-lysine derivative peak area and concentration extended from 1 to 200 mu g L-1. The limit of detection was 1 mg kg(-1), and the limit of quantification was 5 mg kg(-1). The average recoveries ranged from 100% to 104% at 10, 150 and 350 mg kg(-1) spiked levels, and the relative standard deviation was within 5% (n = 5).

• 出版日期2015-8
• 单位中国水产科学研究院; 中国海洋大学