Analysis of Fatty Acid Compositions in Body Wall and Internal Organs of Sea Cucumber

作者：Lou Qiaoming; Li Laihao; Chen Shengjun; Yang Wenge; Zhang Jinjie; Xue Changhu

来源：Journal of Chinese Institute of Food Science and Technology, 2017, 17(7): 221-229.

DOI：10.16429/j.1009-7848.2017.07.028

• Apostichopus japonicas Selenka
• Body walls
• Comprehensive analysis
• Development potential
• Equivalent chain lengths
• Fatty acid composition
• Fatty acid methyl ester
• Internal organs

The fatty acid compositions of body wall and internal organs of Apostichopus japonicas Selenka were analyzed by gas chromagraphy-mass spectrometry (GC-MS), after derivatization with methyl-esterification and dimethyl disulfide. The results indicated that the basic types of fatty acid methyl esters could be firstly confirmed by base-peak ions, according to the fragmentation rules and mass spectrometry characteristics, and then the comprehensive analyses were made on the basis of characteristic ions and equivalent chain length (ECL). Fifty fatty acids and six fatty aldehyde dimethyl acetals were identified from Apostichopus japonicas Selenka, and the principal fatty acids were successively C16:0, C16:1n-9, C20:1n-11, C20:5n-3, C18:0DMA, C20:4n-6and C23:1n-9, and C23:1n-9was characteristic fatty acid of sea cucumbers. Meanwhile, significant differences were demonstrated between body wall and internal organs of Apostichopus japonicas Selenka. The contents of polyunsaturated fatty acids and fatty aldehyde dimethyl acetals were respectively 21.32% and 9.97% in body wall, and were remarkably higher than those in internal organs (9.93% and 3.06%, respectively);nevertheless, the content of saturated fatty acids (24.55%) was significantly lower than that in internal organs (39.54%). Monounsaturated fatty acids were based on n-9 type and n-11 type, and the contents were respectively 44.91% in body wall and 47.47% in internal organs. The content of n-9 type monounsaturated fatty acids was 22.92% in body wall and was dramatically lower than that in internal organs (31.57%);however, the content of n-11 type was 10.73% and significantly higher than that in internal organs (4.75%). The body wall and internal organs of Apostichopus japonicas Selenka were rich in monounsaturated fatty acids, especially n-9 type monounsaturated fatty acids;moreover, C20:5n-3, C20:4n-6and plasmalogens were found abundantly in body wall, indicating that Apostichopus japonicas Selenka had high nutritional values in lipids and fatty acids and also could be used as important dietary sources of functional lipid factors, and had great development potentials of functional food.

• 出版日期2017