Direct in vivo measurement of glycine and the neurochemical profile in the rat medulla oblongata

作者：Xin Lijing^*; Gambarota Giulio; Duarte Joao M N; Mlynarik Vladimir; Gruetter Rolf

来源：NMR in Biomedicine, 2010, 23(9): 1097-1102.

DOI：10.1002/nbm.1537

• glycine
• neurochemical profile
• medulla oblongata
• NMR spectroscopy

The medulla oblongata (MO) contains a high density of glycinergic synapses and a particularly high concentration of glycine The aims of this study were to measure directly in vivo the neurochemical profile, including glycine, in MO using a spin-echo-based (1)H MRS sequence at TE = 2 8 ms and to compare it with three other brain regions (cortex, striatum and hippocampus) in the rat Glycine was quantified in MO at TE = 2 8 ms with a Cramer-Rao lower bound (CRIB) of approximately 5% As a result of the relatively low level of glycine in the other three regions, the measurement of glycine was performed at TE = 20 ms, which provides a favorable J-modulation of overlapping myo-inositol resonance The other 14 metabolites composing the neurochemical profile were quantified in vivo in MO with CRLBs below 25% Absolute concentrations of metabolites in MO, such as glutamate, glutamine, gamma-aminobutyrate, taurine and glycine, were in the range of previous in vitro quantifications in tissue extracts Compared with the other regions, MO had a three-fold higher glycine concentration, and was characterised by reduced (p < 0 001) concentrations of glutamate (-50 +/- 4%), glutamine (-54 +

• 出版日期2010-11