A simple method for measuring excess adsorption isotherms of organic eluent components on reversed-phase packing materials

作者：Ohashi Junji^*; Harada Makoto; Okada Tetsuo

来源：Journal of Separation Science, 2017, 40(4): 842-848.

DOI：10.1002/jssc.201601032

• excess adsorption isotherms
• frontal analysis
• refractive index
• reversed-phase liquid chromatography
• staircase mode

A simple frontal analysis method has been developed for the reliable measurement of excess adsorption isotherms of an organic component on reversed-phase adsorbents in a series of programmed concentration steps. In the present method, a peak, which is produced by refractive index change in column eluate, is detected at 589 nm; it represents the elution volume of the boundary. The method is applied to the measurement of the excess adsorption isotherms of organic eluent components from water on commercially available reversed-phase stationary phases. The results are in good agreement with the previously reported isotherms. We also measure the excess adsorption isotherms of organic eluent components from solutions containing electrolytes. There are not any interference peaks on the elution traces. The method is thus reliably applicable to the evaluation of the excess adsorption of organic eluent components in practical systems.

• 出版日期2017-2