Molecular cloning, sequence analysis and tissue expression of translationally controlled tumour protein from the WSSV-infected Indian shrimp Penaeus indicus

作者：Rajesh Subramanian; Kiruthiga C; Rashika Vittobai; Priya Revathi; Narayanan Rangarajan Badra^*

来源：Aquaculture Research, 2010, 41(4): 545-551.

DOI：10.1111/j.1365-2109.2009.02348.x

• WSSV
• Penaeus indicus
• TCTP
• haemocytes

The gene coding for translationally controlled tumour protein (TCTP) was polymerase chain reaction amplified from haemocyte cDNA of Indian shrimp, Penaeus indicus, and sequenced. The N-terminal region, a conserved one among all the TCTPs, was shown to have one substitution at position 37, in the Indian isolate. Besides this, there were two substitutions in the C-terminal region (135, 149), exclusive to the Indian isolate. Phylogenetic analysis suggested a close relatedness of TCTP from P. indicus to Fenneropenaeus chinensis compared with other isolates. Translationally controlled tumour protein gene expression was found to be elevated in the haemocytes of WSSV-infected shrimps compared with the uninfected ones. However, tissues from the infected shrimps did not exhibit any detectable levels of TCTP expression.

• 出版日期2010-3-17