delta-Opioid receptors stimulate ERK1/2 activity in NG108-15 hybrid cells by integrin-mediated transactivation of TrkA receptors

作者：Eisinger Daniela A^*; Ammer Hermann

来源：FEBS Letters, 2008, 582(23-24): 3325-3329.

DOI：10.1016/j.febslet.2008.08.021

• Cell adhesion
• delta-Opioid receptor
• Integrin
• Extracellular signal-regulated protein kinase
• Tropomyosin-related kinase A

This study demonstrates that activation of delta-opioid receptors (DORs) in neuroblastoma x glioma (NG108-15) hybrid cells by [D-Ala(2), D-Leu(5)] enkephalin (DADLE) and etorphine significantly enhances cell adhesion to fibronectin-coated wells. This effect is blocked by both naloxone and integrin binding RGDT peptides. In addition, cell adhesion turned out to be a prerequisite for DOR-stimulated transactivation of Tropomyosin-related kinase A (TrkA) and extracellular signal-regulated kinases 1/2 (ERK1/2). Because inhibition of TrkA activation by AG879 completely blocked DOR- and integrin-mediated ERK1/2 signaling, the present results indicate that in NG10815 cells DOR- stimulated ERK1/2 activation is mediated by integrin-induced transactivation of TrkA.

• 出版日期2008-10-15