Detection of high-risk human papillomavirus type 16 and 18 using isothermal helicase-dependent amplification

作者：Barbieri Daniela; Venturoli Simona; Roesl Fiank; Rincon Orozco Bladimiro

来源：Diagnostic Microbiology and Infectious Disease, 2014, 79(2): 178-182.

DOI：10.1016/j.diagmicrobio.2014.02.012

• HPV detection
• Isothermal helicase-dependent amplification
• Cost-effective method
• Cervical cancer

Persistent infection with human papillomavirus (HPV) induces cervical cancer. Here, we describe a sensitive, specific, and rapid assay for high-risk HPV16 and 18 detection by isothermal helicase-dependent amplification. This method can be used as cost-effective diagnostic method for low-income countries, where highest incidences worldwide of cervical cancer are registered.

• 出版日期2014-6