MPF-based meiotic cell cycle control: Half a century of lessons from starfish oocytes

作者：Kishimoto Takeo^*

来源：Proceedings of the Japan Academy Series B-Physical and Biological Sciences, 2018, 94(4): 180-203.

DOI：10.2183/pjab.94.013

• cell cycle
• meiosis
• M-phase
• MPF
• cyclin B-Cdkl
• oocyte

In metazoans that undergo sexual reproduction, genomic inheritance is ensured by two distinct types of cell cycle, mitosis and meiosis. Mitosis maintains the genomic ploidy in somatic cells reproducing within a generation, whereas meiosis reduces by half the ploidy in germ cells to prepare for successive generations. The meiotic cell cycle is believed to be a derived form of the mitotic cell cycle; however, the molecular mechanisms underlying both of these processes remain elusive. My laboratory has long studied the meiotic cell cycle in starfish oocytes, particularly the control of meiotic M-phase by maturation- or M phase-promoting factor (MPF) and the kinase cyclin B-associated Cdkl (cyclin B-Cdkl). Using this system, we have unraveled the molecular principles conserved in metazoans that modify M-phase progression from the mitotic type to the meiotic type needed to produce a haploid genome. Furthermore, we have solved a long-standing enigma concerning the molecular identity of MPF, a universal inducer of M-phase both in mitosis and meiosis of eukaryotic cells.

• 出版日期2018-4