Hybridization biosensor using diaquabis[N-(2-pyridinylmethyl)benzamide-kappa N-2,O]-cadmium(II) dinitrate as a new electroactive indicator for detection of human hepatitis B virus DNA

作者：Zhang Shusheng^*; Tan Qianqian; Li Feng; Zhang Xiaoru

来源：Sensors and Actuators B: Chemical , 2007, 124(2): 290-296.

DOI：10.1016/j.snb.2006.12.040

• electrochemical DNA biosensor
• hepatitis B virus
• hybridization
• voltammetry
• [CdL2](2+)

The complex diaquabis[N-(2-pyridinylmethyl) benzamide-kappa(2) N,O]-cadmium(II) dinitrate {[CdL2(H2O)(2)](NO3)(2), where L=N-(2-pyridinylmethyl) benzamide} was synthesized and characterized by X-ray diffraction analysis. Fluorescence spectroscopy and voltammetry were used to probe the interaction between [CdL2](2+), and salmon sperm DNA. Results showed that [CdL2](2+) had excellent electrochemical activity on glassy carbon electrode (GCE) and could intercalate into the double helix of double-stranded DNA (dsDNA). In 0.2 mol L-1 NaAc-HAc media (pH 7.02), the binding ratio between [CdL2](2+) and salmon sperm DNA was calculated to be 2:1 and the binding constant was 25.56L(1/2) mol(-1/2). An electrochemical DNA biosensor for the detection of human hepatitis B virus (HBV) DNA fragment was developed. The biosensor relied on the covalent immobilization of the 21-mer single-stranded DNA (ssDNA) related to HBV gene on the modified glassy carbon electrode (GCE). Using [CdL2](2+) as novel electroactive indicator, the hybridization between the probe and its complementary ssDNA, as the target, was investigated by differential pulse voltammetry (DPV). Experiment with non-complementary oligonucleotides was carried out to assess the selectivity of the developed electrochemical DNA biosensor. The target HBV DNA could be quantified ranged from 1.01 x 10(-8) to 1.62 x 10(-6) mol L-1 with good linearity (gamma = 0.9962). The detection limit was 7.19 x 10(-9) mol L-1 (3 sigma, n = 11).

• 出版日期2007-6-26
• 单位青岛科技大学