RAPID GENOTYPE ANALYSIS IN TYPE 2B VON WILLEBRANDS DISEASE USING A UNIVERSAL HETERODUPLEX GENERATOR

作者：WOOD N; STANDEN GR; MURRAY EW; LILLICRAP D; HOLMBERG L; PEAKE IR; BIDWELL J

来源：British Journal of Haematology, 1995, 89(1): 152-156.

• VON WILLEBRANDS DISEASE
• TYPE 2B
• GENOTYPE
• MUTATIONS
• HETERODUPLEX

A new diagnostic technique based on DNA heteroduplex analysis has been used to identify specific point mutations in the von Willebrand's factor (vWF) gene of patients with von Willebrand's disease type 2B. Molecular analysis in these patients has shown previously that their mutations are clustered in a short region of sequence in exon 28 of the VWF gene. The principle of the method involves heteroduplex formation between amplified genomic sequence containing the defect and an exon 28 vWF gene universal heteroduplex generator (UHG). The UHG is a synthetic VWF gene exon 28 homologue which contains a specific heteroduplexes for each type 2B mutation. Individual mutant genotypes are identified by characteristic banding patterns following polyacrylamide minigel electrophoresis. The technique is rapid, simple, inexpensive, and is ideally suited for adoption by non-specialist haematology laboratories for screening purposes.

• 出版日期1995-1