Molecular Investigations of a Locally Acquired Case of Melioidosis in Southern AZ, USA

作者：Engelthaler David M^*; Bowers Jolene; Schupp James A; Pearson Talima; Ginther Jennifer; Hornstra Heidie M; Dale Julia; Stewart Tasha; Sunenshine Rebecca; Waddell Victor; Levy Craig; Gillece John; Price Lance B; Contente Tania; Beckstrom Sternberg Stephen M; Blaney David D; Wagner David M; Mayo Mark; Currie Bart J; Keim Paul; Tuanyok Apichai

来源：PLoS Neglected Tropical Diseases, 2011, 5(10): e1347.

DOI：10.1371/journal.pntd.0001347

Melioidosis is caused by Burkholderia pseudomallei, a Gram-negative bacillus, primarily found in soils in Southeast Asia and northern Australia. A recent case of melioidosis in non-endemic Arizona was determined to be the result of locally acquired infection, as the patient had no travel history to endemic regions and no previous history of disease. Diagnosis of the case was confirmed through multiple microbiologic and molecular techniques. To enhance the epidemiological analysis, we conducted several molecular genotyping procedures, including multi-locus sequence typing, SNP-profiling, and whole genome sequence typing. Each technique has different molecular epidemiologic advantages, all of which provided evidence that the infecting strain was most similar to those found in Southeast Asia, possibly originating in, or around, Malaysia. Advancements in new typing technologies provide genotyping resolution not previously available to public health investigators, allowing for more accurate source identification.

• 出版日期2011-10