科研之友
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摘要
Pertactin is a surface adhesin of Bordetella pertussis which is produced in small quantities when expressed from the native pm promoter. Hybrid genes were constructed in which the pm promoter was replaced by either the fha or tox promoter. Recombinant B. pertussis strains containing chromosomally integrated hybrid tox promoter/prn (toxpprn) or fha promoter/prn (fhapprn) genes expressed pertactin at similar to 5- and 8-fold the wild-type level, respectively. The pertactin was correctly processed and secreted and was biochemically and antigenically comparable to its wild-type counterpart, as determined by N-terminal sequence analysis, immunoblotting, peptide mapping, circular dichroism and antigenicity studies. In an adherence assay, a strain over-expressing pertactin was Mo more adherent than the wild-type sb ain, but a pertactin-deficient strain was less adherent.
- 出版日期1995-4
