Amino acid taste receptor regulates insulin secretion in pancreatic beta-cell line MIN6 cells

作者：Oya Manami; Suzuki Hideyuki; Watanabe Yuichiro; Sato Moritoshi; Tsuboi Takashi^*

来源：Genes to Cells, 2011, 16(5): 608-616.

DOI：10.1111/j.1365-2443.2011.01509.x

Amino acids such as l-glutamate and l-arginine are potent stimuli for insulin secretion from pancreatic beta-cells. However, the precise molecular mechanisms of amino acid-induced insulin secretion have only partly understood. In this study, we show here that mouse pancreatic beta-cell line MIN6 cells expressed amino acid taste receptor (heterodimer of type 1 taste G protein-coupled receptor member 1 and member 3; Tas1R1 and Tas1R3, respectively). We found that administration of l-glutamate or l-arginine to MIN6 cells caused the increase in free intracellular concentrations of both inositol-1,4,5-triphosphate (IP(3)) and Ca2+, and umami substance inocinate enhanced the effects of l-glutamate. Effects of amino acids on intracellular IP(3) and Ca2+ concentration were diminished by application of lactisole, a Tas1R3 receptor antagonist. Furthermore, we investigated the effect of amino acids on the insulin release from MIN6 cells by both ELISA and total internal reflection fluorescence microscopy. Application of l-glutamate or l-arginine significantly increased the release of insulin, whereas inhibited by the application of lactisole. Based on these findings, we propose that heterodimer of Tas1R1 and Tas1R3 is the fundamental receptor for the sensing amino acids and regulates the amino acid-induced insulin secretion in pancreatic beta-cells.

• 出版日期2011-5