Novel PCR primers for detection of genetically diverse virulent Agrobacterium tumefaciens biovar 1 strains

作者：Yakabe Lani E; Maccree M Malendia; Sudarshana Padma; McClean Ali E; Parker Shane R; Wechter William P; Presting Gernot; Marutani Hert Mizuri; Kluepfel Daniel A^*

来源：Journal of General Plant Pathology, 2012, 78(2): 121-126.

DOI：10.1007/s10327-012-0364-z

• Agrobacterium tumefaciens
• Crown gall
• Species-specific
• Virulence-specific
• PCR detection
• Ti-plasmid

Novel PCR primers were developed to amplify a 243-bp fragment of an intergenic region between gene 5 and tms2 on the T-DNA of Agrobacterium tumefaciens biovar 1. These primers exhibit 100% positive correlation with strain virulence, 100% negative correlation with avirulence, and did not generate extraneous bands, thus facilitating robust real-time PCR detection.

• 出版日期2012-3