An electrochemical investigation of hemoglobin and catalase incorporated in collagen films

作者：Li M; He PL; Zhang Y; Hu NF^*

来源：Biochimica et Biophysica Acta-Proteins and Proteomics, 2005, 1749(1): 43-51.

DOI：10.1016/j.bbapap.2005.02.002

• hemoglobin
• catalase
• collagen
• direct electrochemistry
• electrochemical catalysis

Collagen, an electrochemically inert protein, formed films on pyrolytic graphite (PG) electrodes, which provided a suitable microenvironment for heme proteins to transfer electron directly with the underlying electrodes. Hemoglobin (Hb) and catalase (Cat) incorporated in collagen films exhibited a pair of well-defined and quasi-reversible cyclic voltammetric peaks at around -0.35 V and -0.47 V (vs. SCE) in pH 7.0 buffers, respectively, characteristic of the protein heme Fe(III)/Fe(II) redox couples. UV-vis spectra showed that the heme proteins in collagen films retained their near-native conformations in the medium pH range. The results of scanning electron microscopy (SEM) demonstrated that the interaction between heme proteins and collagen made the morphology of dry protein-collagen films different from the collagen films alone. The electrochemical parameters such as apparent heterogeneous electron transfer rate constant (k(s)) and formal potential (E-ol) of the films were estimated by using square wave voltammograms (SWV) and nonlinear regression analysis. The heme protein-collagen film electrodes were also used to catalyze the reduction of nitrite, oxygen and hydrogen peroxide, indicating potential applications of the films for the fabrication of a new type of biosensor that does not use mediators. © 2005 Elsevier B.V.

• 出版日期2005-5-20
• 单位北京师范大学; 中国兵器工业集团第七〇研究所