科研之友
微信
新浪微博
Facebook
分享链接
摘要
Aggregates of amyloid beta (A) peptides are believed to be responsible for the neuropathology of Alzheimer's disease. In this work, ferrocene (Fc) is attached to the aggregating core of the A peptides, KLVFFAE. Inhibition of Fc-KLVFFAE aggregation by curcumin, a natural compound, was monitored by HPLC-electrochemical detection (HPLC-EC). The Fc oxidation current is dependent on the incubation condition and curcumin can retain Fc-KLVFFAE in its monomeric form. We demonstrate that tagging Fc to KLVFFAE affords a cost-effective and electroactive mimicry of A(142) and HPLC-EC is suitable for sensitive, reproducible, and facile screening of drugs for inhibiting the aggregation of A peptides.
- 出版日期2013-7
