Differential role of YiGanKang Decoction in IL-1 beta induction of IL-1RI and AP-1 in rat hepatic stellate cell

作者：Zhang, Yaping^*; Ai, Xuemei; Zhang, Junxia; Yao, Xixian

来源：Journal of Ethnopharmacology, 2012, 141(2): 599-602.

DOI：10.1016/j.jep.2011.09.001

• YiGanKang Decoction
• Interleukin-1 beta
• Type I receptor
• Activator protein 1
• Hepatic stellate cells

Background/Aim: YiGanKang, a combination of Chinese herbs, has anti-fibrosis effects in chronic liver diseases. The present study tries to demonstrate differential role of YiGanKang Decoction in interleukin-1 beta (IL-1 beta) induction of the type I receptor (1L-1RI) and the activator protein 1 (AP-1) in rat hepatic stellate cell (HSC).
Methods: Flow cytometry was used to detect the IL-1RI expression. Electrophoretic mobility shift assays was used to detect AP-1 activity.
Results: IL-1RI expression after IL-1 beta treatment for 0, 2, 10, 60, and 120 min was 227.08 (13.15), 268.43 (8.93), 442.97 (7.00), 367.66 (14.70), and 261.58 (15.08), respectively. The differences between IL-1RI expression after treatment for 10 and 60 min were significantly higher than the corresponding values in the control (P<0.01, P<0.01, respectively); After pretreatment with YiGanKang Decoction, IL-1RI expression induced by 1L-1 beta was not decrease obviously; IL-1 beta could activate AP-1 in rat HSCs (P<0.01). Meanwhile YiGanKang Decoction could inhibit activity of AP-1 induced by IL-1 beta (P<0.01), and the inhibition rate was 42.71%.
Conclusion: YiGanKang Decoction could not decrease IL-1RI expression, but it could inhibit activity of AP-1 in rat HSCs induced by IL-1 beta.

• 出版日期2012-6-1
• 单位河北医科大学