Abiotic stress of ambient cold temperature regulates the host receptivity to pathogens by cell surfaced sialic acids

作者：Moon Seong Cheol; Joo Su Yeon; Chung Tae Wook; Choi Hee Jung; Park Mi Ju; Choi Hee Jin; Bae Sung Jin; Kim Keuk Jun; Kim Cheorl Ho; Joo Myungsoo; Ha Ki Tae^*

来源：Biochemical and Biophysical Research Communications, 2016, 476(3): 159-166.

DOI：10.1016/j.bbrc.2016.05.020

• Cold temperature
• Sialic acid
• Sialyltransferase
• Hemagglutinin
• Virus
• Receptivity

Ambient cold temperature, as an abiotic stress, regulates the survival, stability, transmission, and infection of pathogens. However, the effect of cold temperature on the host receptivity to the pathogens has not been fully studied. In this study, the expression of terminal alpha-2,3- and alpha-2,6-sialic acids were increased in murine lung tissues, especially bronchial epithelium, by exposure to cold condition. The expression of several sialyltransferases were also increased by exposure to cold temperature. Furthermore, in human bronchial epithelial BEAS-2B cells, the expressions of alpha-2,3- and alpha-2,6-sialic acids, and mRNA levels of sialyltransferases were increased in the low temperature condition at 33 degrees C. On the other hand, the treatment of Lith-Gly, a sialyltransferase inhibitor, blocked the cold-induced expression of sialic acids on surface of BEAS-2B cells. The binding of influenza H1N1 hemagglutinin (HA) toward BEAS-2B cells cultured at low temperature condition was increased, compared to 37 degrees C. In contrast, the cold increased HA binding was blocked by treatment of lithocholicglycine and sialyl-N-acetyl-D-lactosamines harboring alpha-2,3- and alpha-2,6-sialy1 motive. These results suggest that the host receptivity to virus at cold temperature results from the expressions of alpha-2,3- and alpha-2,6-sialic acids through the regulation of sialyltransferase expression.

• 出版日期2016-7-29