Molecular characterization of chikungunya virus circulating in urban and rural areas of West Bengal, India after its re-emergence in 2006

作者：Taraphdar Debjani; Chatterjee Shyamalendu^*

来源：Transactions of the Royal Society of Tropical Medicine and Hygiene, 2015, 109(3): 197-202.

DOI：10.1093/trstmh/tru166

• Chikungunya virus
• India
• Molecular characterization
• West Bengal

Background: In the state of West Bengal, chikungunya virus (CHIKV) has re-emerged in 2006 after its last occurrence in 1963-1965 in this state. The virus rapidly affected almost every district of this state, with high morbidity. Based on complete sequences of structural region of CHIKV genome, we determined the molecular characterization of the virus circulating in this state from 2006-2012.
Methods: CHIKV was isolated from 20 acute CHIKV RT-PCR positive serum samples in C6/36 mosquito cell line. These samples were collected from 20 patients with a clinical history of <= 2 days of fever and chikungunya-like illness. Those patients were residing in some outbreak areas in the state of West Bengal during 2006-2012. Isolation was confirmed through RT-PCR and sequencing.
Results and Conclusions: Two sub-lineages of East-central-southern African (ECSA) genotype of CHIKV strains were circulating simultaneously in this state during the study period; one type was circulating in rural areas of the state from 2006 whereas another type was isolated from the metropolitan city of Kolkata in 2011 and 2012. The mutational pattern of those CHIKV strains suggests that the transmission of the viruses might be facilitated by different species of Aedes mosquitoes. Our results represent an important first step towards understanding the circulating strains of CHIKV in the state of West Bengal with the geographical variation.

• 出版日期2015-3