Autotoxicity of peanut and identification of phytotoxic substances in rhizosphere soil

作者：Huang, Y. Q.^*; Han, X. R.; Yang, J. F.; Liang, C. H.; Zhan, X. M.

来源：Allelopathy Journal, 2013, 31(2): 297-308.

• Allelopathy
• Arachis hypogaea
• autotoxicity
• continuous cropping
• coumarin
• phenolic acids
• peanut
• vanillic acid

We evaluated the autotoxicity of peanut (Arachis hypogaea L.) leaf, stem and root extracts, and identified the phytotoxic substances accumulated in peanut rhizosphere soil during continuous cropping. We prepared aqueous extracts from peanut leaves, stems, and roots, and evaluated their effects on peanut germination and growth. Soil collected from areas in which peanut had been continuously cultivated for 1, 2, 3, or 4 years was analyzed by HPLC in 2009. Leaf extracts drastically inhibited the seed germination, followed by stem extracts and then root extracts. Root extracts severely inhibited the seedling growth, followed by stem extracts and then leaf extracts. We detected 4-types of phenolic acids (p-hydroxy benzoic acid, vanillic acid, coumaric acid, and coumarin) in peanut rhizosphere soil. After 4-years cropping), the vanillic acid and coumarin contents were 0.289 ug/g dry soil and 0.025 ug/g dry soil, respectively. The phenolic acids concentrations increased during the continuous cropping. After 4-year cropping, the vanillic acid concentration in soil was 12-times more than coumarin. The combined concentration of vanillic acid and coumarin increased during continuous cropping (from 0.067 to 0.314 mu g/g dry soil over 4-years). This may be one reason underlying problems with continuous cropping. Thus peanut is a potentially autotoxic specie.

• 出版日期2013-4
• 单位沈阳农业大学