N,N-dimethylsphingosine, an inhibitor of sphingosine kinase, induces phytoalexin production and hypersensitive cell death of Solanaceae plants without generation of reactive oxygen species

作者：Uruma Sadao; Shibata Yusuke; Takemoto Daigo; Kawakita Kazuhito^*

来源：Journal of General Plant Pathology, 2009, 75(4): 257-266.

DOI：10.1007/s10327-009-0176-y

• Hypersensitive cell death
• Phytoalexin
• Phytophthora infestans
• Potato
• Reactive oxygen species
• N,N-dimethylsphingosine

Plant recognition of elicitors derived from pathogens induces various resistant reactions, including production of reactive oxygen species, hypersensitive cell death and accumulation of phytoalexins. Previously, we isolated a ceramide elicitor from Phytophthora infestans, which activates O(2) (-) production of potato suspension-cultured cells. In this study, we employed nine ceramide-related chemicals to test their elicitor activity. Although, none of the tested chemicals induced O(2) (-) production, N,N-dimethylsphingosine (DMS) induced accumulation of phytoalexin in potato tubers. In potato, tobacco and Nicotiana benthamiana, DMS also induced rapid cell death. DMS-treated potato cells stained with 4',6-diamidino-2-phenylindole (DAPI) showed chromatin condensation, and isolated DNA from DMS-treated cells had ladder pattern, confirming that DMS-induced plant cell death is a hypersensitive reaction-like programmed cell death. Involvement of ceramide signaling in induction of plant defense reactions is discussed.

• 出版日期2009-8