A highly efficient designer cell for enantioselective reduction of ketones

作者:Srivastava Gautam*; Pal Mohan; Kaur Suneet; Jolly Ravinder S
来源:Catalysis Science & Technology, 2015, 5(1): 105-108.
DOI:10.1039/c4cy01017e

摘要

A designer cell, surf-crs-gdh, coexpressing carbonyl reductase (crs) and glucose dehydrogenase (gdh) on the cell surface has been constructed and its enzyme activities are compared with those of the corresponding cell, cyto-crs-gdh, coexpressing crs and gdh in cytosol. For various ketones, surf-crs-gdh exhibited 48- to 265-fold higher crs activity per unit protein compared to cyto-crs-gdh.

  • 出版日期2015