Na+-driven multidrug efflux pump VcmA from Vibrio cholerae non-O1, a non-halophilic bacterium

作者：Huda MN; Morita Y; Kuroda T; Mizushima T; Tsuchiya T^*

来源：FEMS Microbiology Letters, 2001, 203(2): 235-239.

DOI：10.1111/j.1574-6968.2001.tb10847.x

• drug resistance
• VcmA drug efflux pump
• Na+/drug antiporter
• Vibrio cholerae

A chromosomal DNA fragment from Vibrio cholerae non-O1 containing a drug resistance determinant was cloned and sequenced. The deduced amino acid sequence suggested that the determinant gene encodes a multidrug efflux pump. We designated the pump VcmA. Escherichia coh cells transformed with a plasmid carrying the vcmA gene showed increased resistance against norfloxacin, ciprofloxacin, ofloxacin, daunomycin, doxorubicin, streptomycin, kanamycin, ethidium bromide. 4',6-diamidino-2-phenylindole dihydrochloride, Hoechst 33342 and acriflavine. Na+ (or Li-)-dependent efflux of ethidium bromide was detected in transformant cells. Efflux of Na+, elicited by ethicium bromide, was observed from transformant cells. Thus, we concluded that the VcmA is a Na+/drug antiporter.

• 出版日期2001-9-25